Coding
INP-His

Part:BBa_K1933001:Design

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)


INPNC fused to 6xHis tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 952
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 823
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes

We omitted stop codon from this part to make BBa_K1933011.
We used BBa_K1933011 for construction of ☆BBa_K1933100, BBa_K1933101, ☆BBa_K1933200.

Source

INPNC derived from BBa_K811005.